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. 2017 Nov 22;7:16082. doi: 10.1038/s41598-017-16463-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HFD increases UCP1 protein levels without changes in BAT mitochondrial content. Whole interscapular BAT lysates were subjected to Western Blot analysis. (A) UCP1 protein levels using β-actin as a loading control. (B) Relative mRNA expression of BAT markers Ucp1, Zic1, Prdm16, PGc1α, Bmp8b, Cidea, Dio2, Fgf21, Hif1α and Leptin in NCD and HFD-fed mice. (C) TIM44 protein levels using β-actin as a loading control. Shown representative immunoblots out of at least 4 independent experiments. (D) Mitochondrial dynamics were determined by the relative mRNA expression of Mfn1, Mfn2 and Opa1 of BAT in NCD and HFD-fed mice. Results are represented as mean ± SEM. n = 8–10; *p < 0.05.