. 2017 Oct 23;38(1):32–38. doi: 10.3343/alm.2018.38.1.32

Table 1. Primers used for multiplex PCR and sequencing.

Method	Genomic target	Nucleotide sequence	Product size (bp)	References
Multiplex PCR (set A)	RHD exon 4	F: CCACATGAACATGATGCACA	127	[18]
	RHD exon 4	R: CAAACTGGGTATCGTTGCTG	127	[18]
	RHD exon 10	F: TAAGCAAAAGCATCCAA	186	[6]
	RHD exon 10	R: ATGGTGAGATTCTCCT	186	[6]
Multiplex PCR (set B)	RHD exon 7	F: GTTGTAACCGAGTGCTGGGGATTC	123	[19]
	RHD exon 7	R: TGCCGGCTCCGACGGTATC	123	[19]
	RHD promoter	F: TCCACTTTCCACCTCCCTGC	256	[19]
	RHD promoter	R: GCAGCCAACTTCCCCTGTG	256	[19]
Multiplex PCR (internal control)	HGH	F: TGCCTTCCCAACCATTCCCTTA	434	[13]
Multiplex PCR (internal control)	HGH	R: CCACTCACGGATTTCTGTTGTGTTTC	434	[13]
Sequencing	RHD exon 9	F: AGATACTGTCGTTTTGACACACAAT	463	In this study
Sequencing	RHD exon 9	R: GCTGAGGACTGCAGATAGGG	463	In this study