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. 2017 Nov 22;7:16011. doi: 10.1038/s41598-017-16197-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Semi-quantitative analysis of HMGB1⁺ cells within post-mortem tissues of dengue fatal cases. For each studied organ (liver, lung and heart), 50 fields were acquired under a Nikon ECLIPSE E600 microscope coupled with a Cool SNAP-Procf Color camera. Cells with cytoplasmic HMGB1 expression were quantified using organ samples from four dengue fatal cases and four non-dengue control cases. Results are expressed as the median of HMGB1⁺ cells per 0.96 mm². Mann-Whitney non-parametric statistical test was applied to verify the differences between groups (***p < 0.001).