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. 2017 Nov 22;7:16011. doi: 10.1038/s41598-017-16197-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Co-staining of DENV and HMGB1 in the lung. Lung sections from dengue fatal case (case 1) and a non-dengue case were processed for detection of DENV and HMGB1 as explained in Fig. 5. In this dengue case, DENV RNA was found in alveolar macrophages (AM) and in cells from the alveolar septum. HMGB1 expression occurred within the nucleus as well as the cytoplasmic regions. DENV RNA and HMGB1 signals co-stained within the cytoplasm of cells. In the control case, the HMGB1 expression was marginal and predominantly in nuclear regions.