Fig. 4.

C1q reduction causes defective efferocytosis by Mafb ^−/− macrophages. a Apoptotic Jurkat cells treated with CellTracker were incubated with Mafb ^−/− fetal liver-derived macrophages in 0, 20, and 50% serum from WT mice (0%, n = 4; 20%, n = 3; 50%, n = 4). b Apoptotic Jurkat cells were incubated with Mafb ^−/− macrophages with 50% WT serum and a different time course (n = 4 for each group). c Apoptotic Jurkat cells were incubated with or without heat-inactivated serum or normal serum (serum-, n = 5; inactivated serum, n = 5; serum, n = 3). a–c The data are from one experiment that was representative of at least two independent experiments. d Apoptotic Jurkat cells were incubated with Mafb ^−/− macrophages with or without C1q-deficient serum or WT serum (WT, n = 6; Mafb ^−/−, n = 10 for each sample). e Apoptotic Jurkat cells were incubated with Mafb ^f/f::LysM-Cre fetal liver-derived macrophages that treated with or without 100 μg of purified human C1q 1 h before (Mafb ^f/f, n = 3; Mafb ^f/f::LysM-Cre, n = 10; Mafb ^f/f::LysM-Cre + C1q, n = 10). d, e The results are from two pooled independent experiments. a–e Quantification data are presented as the mean ± s.e.m.; **p < 0.01, n.s. not significant (a–e, Welch’s t-test)