Fig. 5.

ANAs are detected in serum from fetal liver transplanted Mafb ^−/− mice. a Nuclei of HEp-2 cells were stained with mouse serum from WT or Mafb ^−/− fetal liver-transplanted mice using the HEPANA test. A positive signal indicates the presence of ANA. b Table showing ANA-positive numbers using serum diluted 1:60 and collected 4, 8, and 20 weeks after fetal liver transplantation; 4–8 weeks (male: WT, n = 19; Mafb ^−/−, n = 21), 20 weeks (female: WT, n = 12; Mafb ^−/−, n = 15; male: WT, n = 28; Mafb ^−/−, n = 32). *p < 0.05 (Fisher’s exact test). c Serum collected from WT or Mafb ^−/− mice 20 weeks after transplantation was diluted 160-fold, 320-fold, 640-fold, 1280-fold, and 2560-fold (WT, n = 30 (male, n = 19; female, n = 11); Mafb ^−/−, n = 31 (male, n = 17; female, n = 14)). The number of Mafb ^−/− mice with an ANA titer (range: 1:160–1:2560) was significantly higher compared with WT mice (p < 0.05, Fisher’ s exact test). b, c Data from two to four independent experiments were pooled