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. 2017 Nov 22;8:1700. doi: 10.1038/s41467-017-01711-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — MafB critically regulates C1q-dependent gene expression. a Activation of the C1q promoters by MafB or the PPARδ and RXRα complex was compared using a luciferase assay. The data are from one experiment that was representative of two independent experiments. b Empty vector (mock), MafB, c-Maf, PPARδ/RXRα, PPARγ/RXRα, LXRα/RXRα, or RXRα-expressing vectors were transfected into RAW264.7 cells. The expression of Mafb, C1qa, C1qb, and C1qc was analyzed using qRT-PCR (n = 3 for each group). The data are from one experiment that was representative of at least two independent experiments. c Control macrophages were treated with GW0742/9cRA (PPARδ/RXRα agonist), T1317/9cRA (LXRα/RXRα agonist), ATRA (RAR agonist), dexamethasone (glucocorticoid), and INFγ. Mafb expression was analyzed using qRT-PCR (n = 3 for the GW0742, T1317/9cRA, ATRA, and dexamethasone groups; n = 2 for IFNγ). d WT and Mafb ^−/− macrophages were stimulated with GW0742 or T1317/9cRA. Mafb ^f/f and Mafb ^f/f::Tie2-Cre macrophages were stimulated with ATRA, dexamethasone or IFNγ. The expression of C1qa, C1qb, and C1qc was analyzed using qRT-PCR (n = 3 for the GW0742, T1317/9cRA, ATRA, and dexamethasone groups; n = 2 for IFNγ). b–d The expression of target genes was normalized to Hprt mRNA. c, d The data are presented as the mean ± s.e.m. ^*WT vs. Mafb ^−/−, Mafb ^f/f vs. Mafb ^f/f::Tie2-Cre; # no treatment vs. agonist; ^{*, #} p < 0.05, ^{**, ##} p < 0.01, n.s. not significant (Student’s t-test)