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. 2017 Nov 22;7:16010. doi: 10.1038/s41598-017-16255-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pyridoxamine reduces collagen content. (a, upper panel) Representative images of interstitial collagen obtained with Sirius red/Fast Green in the peri-infarct area. (a, lower panel) Representative images of collagen type I and type III, under polarized light microscopy. The red color indicates the highly cross-linked collagen type I, the green color indicates immature collagen type III. (b) Total interstitial collagen quantification in the peri-infarct area. (c) Collagen type I (left panel) and collagen type III (right panel) quantification in the peri-infarct region. Data are shown as median [75^th percentile; 25^th percentile] in Sham (N = 5), MI (N = 5) and MI + PM (N = 6). *Denotes p < 0.05 vs Sham, ^#denotes p < 0.05 vs MI.