Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 22;7:16001. doi: 10.1038/s41598-017-16248-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Naïve mESCs differentiated to a ventral hindbrain fate, show appropriate regional and temporal specification. (A) Schematic of the neural differentiation protocol, illustrating morphogen and small molecules employed, to generate ventral hindbrain progenitors and neurons from naïve PSCs. (B–E) Flow cytometry plots at day 7 and, (F) quantitative analysis at 0 and 7 for Oct4, Nestin, FoxA2 and Pax6, showing appropriate downregulaton of pluripotent (Oct4) and dorsal (Pax6) markers together with upregulation of neural (Nestin) and ventral (FoxA2) expression. (G) Transcriptional expression of hindbrain genes Hoxa1 and Zic1. (H) Representative photomicrographs depicting expression of DAPI, Nestin and Zic1 in hindbrain progenitors at day 7. (I) Ventral hindbrain specification of progenitors was confirmed by the presence of ventral marker FoxA2 and absence of mesodiencephalic protein Otx2. (J) By day 14, hindbrain specified cultures contained serotinergic neurons immunoreactive for TUJ1 and 5HT, as well as (K) Islet1+ TUJ1+ neurons, indicative of motor neurons. Data represents mean ± SEM, (n = 3), Students t-test. Scale bar = 100 um. **p < 0.01, ***p < 0.001.