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. 2017 Nov 22;7:16052. doi: 10.1038/s41598-017-15904-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Simulation of temperature distribution inside the microchamber. (a) Relation between the heating rate for a given bead, B _bead, and the temperature distribution produced by the trap. (b) Simulated temperature profile introducing a 2-μm microsphere at the focus of the trap. The grey area corresponds to the reproducibility of our measurements, shown in Fig. 2a. (MR: melamine resin, PS: polystyrene, Si: silica).