Fig. 4.

Cell cycle arrest and PARP cleavage in ARMS and ERMS cell lines upon ceritinib treatment. a Cells were treated with ceritinib for 24 h and stained with propidium iodide (PI). The level of PI was determined and the percentage of cells in the G0-G1, S, or G2-M phase of the cell cycle was quantified by FACS analysis.*p < 0.05, **p < 0.01, ***p < 0.001. b Effects of 24 h ceritinib treatment on PARP cleavage by Western Blot analysis (PARP = 116 kDa, cleaved PARP = 89 kDa)