Figure 6.
CaM preassociates with NMDA receptors. (A) Both model 1 and model 2 describe well the dependency of CDI on Po (varied by changing glutamate concentrations). (B) (Top) C0 sequence indicates CaM binding region. Residues are color-coded by property relevant to CaM binding: positive charge (blue), negative charge (red), hydrophobic (gray). (Bottom) Whole-cell currents recorded from N1-2aW858A/N2A receptors have altered CDI at all glutamate concentrations tested. (C) (Top) Given here are unitary currents of high-Po mutant N1-2aA652Y, W858A/N2A. (Bottom) Given here are whole-cell currents from the mutants indicated. (D) Shown here is a CDIEQ-Po profile with pooled data from N1-2aA652Y/N2A (black circles) and high-Po double mutant N1-2aA652Y, W858A/N2A (red circle) fit with model 1 (holoCaM association, gray) and model 2 (apoCaM association, blue) (N = 4). To see this figure in color, go online.