Skip to main content
. 2017 Nov 23;37(6):BSR20171229. doi: 10.1042/BSR20171229

Figure 1. Anti-CLN3 antibodies detect the same protein bands in wild-type (WT) and Cln3−/− mouse brain extracts.

Figure 1

Three different anti-CLN3 antibodies [Abnova rabbit anti-CLN3 (D01P), Abnova mouse anti-CLN3 (M03), and Abcam rabbit anti-CLN3 (ab75959)] were tested in immunoblot experiments using protein extracts of surface cross-linked cerebellum and cortex tissue samples from 1-month-old WT and Cln3−/− male mice. Protein extracts were prepared by sonication in a lysis buffer containing 500 mM NaCl, 0.1% NP-40 substitute and protease, and phosphatase inhibitor cocktails. Sixty micrograms of protein were loaded in each lane of SDS-containing 10% polyacrylamide gels. Prior to loading, samples were treated as indicated in reducing sample buffer without or with 4 M urea. After the electrophoretic separation, proteins were transferred onto nitrocellulose membranes and probed with the anti-CLN3 antibodies (Abnova rabbit, 1:500; Abnova mouse, 1:500; Abcam rabbit, 1:700); M.W. marker: PageRuler Plus (Thermo Fisher Scientific). The immunoblots shown are representative of two separate experiments.