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. 2017 Nov 22;61(12):e01388-17. doi: 10.1128/AAC.01388-17

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FIG 3 — Formation of circular forms mediated by IS431. (A) Circular form derived from the region spanning from IS431A to IS431B and the structure that missed the corresponding region of the circular form. (B) Circular form derived from the region spanning from IS431B to IS431C and the structure that missed the corresponding region of the circular form. (C) Structure that missed the region spanning from Δrep to IS431C. The locations and orientations of primers (P1 to P12) are indicated by arrows. Primers P1 to P6 were used for inverse PCR to detect circular forms (primers P3 and P4 for the 12,481-bp circular form and P5 and P6 for the 6,451-bp circular form; inverse PCR with primers P1 and P2 produced no product); primers P7 to P12 were used to detect structures that missed the corresponding region of the circular form (P7 and P8 for structure that missed the region spanning from Δrep to IS431C; P9 and P10 for structure that missed the corresponding region of the 12,481-bp circular form; and P11 and P12 for structure that missed the corresponding region of the 6,451-bp circular form).