FIG 1.

Identification of M. tuberculosis transposon mutants with altered antibiotic susceptibility. (A) Partially inhibitory antibiotic selection of transposon mutant libraries. Growth curves are representative of the growth kinetics of the transposon mutant libraries in the presence or absence of antibiotic selection. d, days. (B) Cluster analysis of CGI profiles. Hierarchical clustering was performed on the CGI profiles derived from triplicate experiments using Pearson's correlation as the similarity metric. The mean log₂ TnSeq-FC in transposon mutant frequency for each gene following antibiotic selection relative to that for the antibiotic-free control is indicated on the color scale, with increased mutant representation being indicated in red and reduced representation being indicated in blue. Genes that did not exhibit statistically significant differences (i.e., q ≥ 0.05, based on resampling testing) in transposon insertion under any of the antibiotic selection conditions tested were omitted, and the 251 remaining genes were used in this analysis.