TABLE 1.
MICs against H37Rv WT, ΔfecB, and fecB-complemented strains
| Antibiotic | Molecular mass (g/mol) | Target | MICa (μg/ml) |
MIC ratiob | ||
|---|---|---|---|---|---|---|
| WT | ΔfecB mutant | fecB-complemented strain | ||||
| Faropenem | 285.3 | Peptidoglycan | 12.5 ± 8.2 | 1.56 ± 1.03 | 21.3 ± 20.5 | 8 |
| Meropenem | 383.5 | Peptidoglycan | 11.3 ± 4.1 | 2.81 ± 1.03 | 11.3 ± 4.1 | 4 |
| Vancomycin | 1449.3 | Peptidoglycan | 20 ± 0 | 1.25 ± 0 | 23.3 ± 8.2 | 16 |
| Fidaxomicin | 1,058.0 | RNA polymerase | 1.2 ± 0.61 | 0.098 ± 0 | 0.8 ± 0.61 | 12 |
| Rifampin | 822.9 | RNA polymerase | 0.02 ± 0 | 0.005 ± 0 | 0.02 ± 0 | 4 |
| Rifabutin | 847.0 | RNA polymerase | 0.01 ± 0 | 0.0029 ± 0.001 | 0.008 ± 0 | 3.4 |
| Ethambutol | 204.3 | Arabinogalactan | 0.91 ± 0 | 0.91 ± 0 | 1.36 ± 0 | 1 |
| Ethionamide | 166.2 | Mycolic acid | 0.55 ± 0.19 | 0.47 ± 0.24 | 0.55 ± 0.19 | 1.2 |
| Isoniazid | 137.1 | Mycolic acid | 0.05 ± 0.011 | 0.05 ± 0.011 | 0.05 ± 0.011 | 1 |
| PA-824 | 359.3 | Mycolic acid | 0.25 ± 0.21 | 0.16 ± 0 | 0.16 ± 0 | 1.6 |
| SQ109 | 330.6 | Mycolic acid | 2.5 ± 0 | 0.63 ± 0 | 2.5 ± 0 | 4 |
| Triclosan | 289.5 | Mycolic acid | 62.5 ± 0 | 62.5 ± 0 | 62.5 ± 0 | 1 |
| Moxifloxacin | 401.4 | DNA gyrase | 0.1 ± 0 | 0.1 ± 0 | 0.1 ± 0 | 1 |
| Chloramphenicol | 323.1 | Protein synthesis | 5.86 ± 0 | 5.86 ± 0 | 8.79 ± 7.18 | 1 |
| Linezolid | 337.4 | Protein synthesis | 1.37 ± 0.48 | 0.39 ± 0 | 1.37 ± 0.48 | 3.5 |
| Streptomycin | 581.6 | Protein synthesis | 0.29 ± 0 | 0.27 ± 0.06 | 0.27 ± 0.06 | 1.1 |
| Nigericin | 725.0 | Respiration | 6.25 ± 4.84 | 1.95 ± 1.28 | 6.25 ± 4.84 | 3.2 |
| Thioridazine | 370.6 | Respiration | 23.4 ± 19.1 | 15.6 ± 0 | 15.6 ± 0 | 1.5 |
| Valinomycin | 1,111.3 | Respiration | 1.56 ± 0 | 0.2 ± 0.15 | 2.34 ± 1.91 | 8 |
a
The mean ± SD MIC values were derived from two independent experiments with triplicate cultures. The MICs were determined as described in Materials and Methods.
b
MIC for WT/MIC for knockout mutant.