FIG 1.

Primers and schematic representations of K13 and mdr1 nested PCR and sequencing strategies. New nested PCR and sequencing primers (A) were developed to capture the full-length K13 propeller region (B) and the 5 haplotype-defining mdr1 SNPs (C). The primers in the table are color-coded to match their positions in the schematics. Positions of reported K13 propeller region SNPs (reviewed in reference 13) are marked in red. Positions of the 5 mdr1 haplotype SNPs (described in reference 48) are marked in black. Schematics were generated using SeqBuilder v.14 (DNASTAR).