FIG 1.

The MvfR inhibitor M64 interferes with P. aeruginosa biofilm formation. Biofilm biomass (a, b, and c) or biofilm viable cells (d and e) were measured by crystal violet staining or CFU counts, respectively, after 24 (a to e), 48, and 72 (e) hours of growth in the presence or absence of 0.1, 1, or 10 μM M64 (a to c and e) or 10 μM M65 (b). Untreated PA14 (a, b, d, and e) or PAO1 (c) biofilms are shown in black, M64-treated biofilms in green, and mvfR− biofilms in red. Shown is the average +/− standard error of the mean (SEM) of at least 5 replicates. Statistical significance was assessed using one-way analysis of variance (ANOVA) plus Dunnett's posttest for multiple comparisons to PA14 or unpaired t test for single comparisons to mvfR− or PAO1. **, P < 0.01; ***, P < 0.001; ns, not statistically significant; -, not treated.