TABLE 1.
qRT-PCR analysis of growth phase-indicating genesa
| Sample | Mean fold change ± SEM in transcript levels relative to PAO1 grown to exponential phase |
||||
|---|---|---|---|---|---|
| PA1137 | piv | tpiA | fdhA | prs | |
| PAO1 (EP) | 1 | 1 | 1 | 1 | 1 |
| PAO1 (SP) | 3.02 ±0.84b | 31.9 ± 15.2b | 0.262 ± 0.11b | 4.31 ± 1.35b | 0.259 ± 0.88b |
| Glutamate-dispersed cells | 14.5 ± 9.4b | 6.33 ± 4.98b | 0.177 ± 0.21b | 6.11 ± 3.26b | 0.235 ± 0.14b |
| Glutamate-dispersed cells, after 2 h of recovery | 16.7 ± 10.0b | 2.60 ± 0.42b | 0.666 ± 0.15 | 7.3 ± 1.44b | 0.557 ± 0.16 |
a
Transcript levels of PA1137, piv, tpiA, fdhA, and prs present in P. aeruginosa PAO1 dispersed cells obtained in response to glutamate and NO, and PAO1 grown planktonically to the exponential (EP) or stationary (SP) phase. The transcript levels are relative to P. aeruginosa PAO1 grown to the exponential phase. Experiments were carried out five times. The transcript levels of mreB were used as a control.
b
Significantly different from PAO1 grown planktonically to exponential phase (P < 0.01 as determined by ANOVA and SigmaStat).