TABLE 2.
qRT-PCR analysis of genes involved in LPS modificationa
| Sample | Mean fold change ± SEM in transcript levels relative to PAO1/pMJT1 grown to exponential phase |
|||
|---|---|---|---|---|
| arnB | arnE | PA4773 | PA4774 | |
| PAO1 (EP) | 1 | 1 | 1 | 1 |
| PAO1 (SP) | ND | −3.74 ± 0.09b | −7.65 ± 0.03b | −2.88 ±0.05b |
| PAO1/pMJT-dipA (EP) | 1.61 ± 0.22 | 1.97 ± 0.12 | 2.81 ± 0.25b | 1.55 ± 0.26 |
| PAO1/pMJT-dipA (SP) | 1.15 ± 0.09 | 1.33 ± 0.24 | 1.59 ± 0.32) | 1.46 ± 0.09 |
| PAO1/pMJT-PA2133 (EP) | 1.11 ± 0.07 | 1.08 ± 0.05 | 1.49 ± 0.22 | 1.26 ± 0.11 |
| PAO1/pMJT-PA2133 (SP) | 1.77 ± 0.33 | 1.28 ± 0.06 | 1.46 ± 0.08 | 1.24 ± 0.15 |
a
Transcript levels of arnB, arnE, PA4773, and PA4774 present in P. aeruginosa PAO1 dispersed cells obtained in response to glutamate and NO, as well as wild-type and PAO1/pMJT-dipA, and PAO1/pMJT-PA2133 strains grown planktonically to exponential (EP) or stationary (SP) phase. The transcript levels are relative to P. aeruginosa PAO1 or PAO1/pMJT1 grown to the exponential phase. Experiments were carried out five times. The transcript levels of mreB were used as a control.
b
Significantly different from PAO1 grown planktonically to the exponential phase (P < 0.01 as determined by ANOVA and SigmaStat). ND, not determined.