TABLE 4.
Primers used in this study
| Method and oligonucleotide | Sequence (5′–3′)a |
|---|---|
| qRT-PCR | |
| arnB-RT-F | ACTGGACTTTCTGCCATTCT |
| arnB-RT-R | GTTCGAGCTCCTGGTTCTT |
| arnE-RT-F | AGCTCACCGTGGAGCACT |
| arnE-RT-R | TGACCAGGACGAAGTTCA |
| PA4773-RT-F | ACTGGTCATCGAGCTGTTCG |
| PA4773-RT-R | CGTACTCCGGCCAGGTATG |
| PA4774-RT-F | CCGCCGAACCACTTCTATTTCT |
| PA4774-RT-R | GCGGTCGTAGTTGTAGGTGTC |
| PA1137-RT-F | GCGAAAATCTTCACCACGAT |
| PA1137-RT-R | CACTTCCTCGACGAAATCCT |
| piv-RT-F | GTCAACCGTCCCTACTGGAG |
| piv-RT-R | GGAGTCGGCGAAATACGATA |
| tpiA-RT-F | CCGGTAACTGGAAAATGCAC |
| tpiA-RT-R | GATGACCTGGCTGATGAACA |
| fdhA-RT-F | TACCTGCAAGGAACAGCACA |
| fdhA-RT-R | GTAGGGCACCAGCACGTATT |
| prs-RT-F | AGTTGGCAAATTCTCCGATG |
| prs-RT-R | CATCACCACCAGTTCCATCA |
| Tn-Check | |
| arnB-RT2_F | GTGGTGATCCCGGTCTAC |
| PhoA_R | CGGGTGCAGTAATATCGCCCT |
| Cloning | |
| PA2133-F-SacI | GCGCGCGCGAGCTCGTGAACGGTTCCCCACAG |
| PA2133-R-EcoRI | GCGCGCGCGAATTCTCACCCCTGGCGGC |
| PA0861_pET-for | CACCATGAGGCAGAACCGG |
| PA0861_pET-rev | CCGGAGGTTCTGTCC |
| PA0861_XbaI-for | GCGCGCTCTAGAATGAGGCAGAACCGGAC |
| PA0861_V5/His_EcoRI-rev | GCGCGCGAATTCTCAATGGTGATGGTGATG |
| PA3343_pET_for | CACCGTGTGCGTGACACA |
| PA3343_pET_rev | GGCCACCACCTGGTTACG |
| PA3343_XbaI_for | GCGCGCTCTAGAGTGTGCGTGACACAGAAG |
| PA3343_V5/His_EcoRI_rev | GCGCGCGAATTCTCAATGGTGATGGTGATG |
a
Restriction sites are underlined.