TABLE 1.
Results of fitting the model to viability dataa
| Parameter (unit) | Estimate | SE | 95% CI |
|
|---|---|---|---|---|
| Model based | Parametric bootstrap | |||
| Early ring stage | ||||
| λ (h−1) | 6.2504 (0.11) | 0.5745 | 5.1243, 7.3765 | 1.8587, 9.5201 |
| α (h−1) | 1.6915 | 0.1378 | 1.4215, 1.9616 | 1.2944, 1.8475 |
| β1 (nM) | 990.84 | 373.49 | 258.81, 1,722.9 | −20,377, 1876.5 |
| β2 (nM) | 12.519 | 1.0631 | 10.435, 14.602 | 10.560, 13.553 |
| Mid-ring stage | ||||
| λ (h−1) | 0.3729 (1.86) | 0.1406 | 0.0974, 0.6485 | 0.2515, 0.5035 |
| α (h−1) | 1.1224 | 0.2455 | 0.6412, 1.6036 | 0.6934, 1.3371 |
| β1 (nM) | 224.39 | 112.12 | 4.6466, 444.14 | 117.37, 301.69 |
| β2 (nM) | 9.97 × 10−4 | 1.26 × 10−4 | (7.5, 12.4) × 10−4 | (9.8, 10.1) × 10−4 |
| Early trophozoite stage | ||||
| λ (h−1) | 1.2290 (0.56) | 0.2249 | 0.7882, 1.6698 | 0.6331, 1.8059 |
| α (h−1) | 5.7434 | 0.7460 | 4.2813, 7.2054 | 1.8799, 7.2729 |
| β1 (nM) | 317.64 | 86.143 | 148.80, 486.48 | −9.4144, 462.98 |
| β2 (nM) | 39.570 | 4.6038 | 30.546, 48.593 | 29.315, 46.842 |
| Late trophozoite stage | ||||
| λ (h−1) | 2.0906 (0.33) | 0.2909 | 1.5203, 2.6608 | 1.4406, 2.6302 |
| α (h−1) | 2.8626 | 0.1591 | 2.5508, 3.1744 | 2.2851, 3.2810 |
| β1 (nM) | 740.02 | 178.77 | 389.64, 1,090.41 | 160.00, 1,071.6 |
| β2 (nM) | 41.405 | 3.6606 | 34.230, 48.580 | 35.459, 45.629 |
a
Viability data are for the 3D7 strain (7). γ is fixed to be 1.7892 based on estimates in Table S1 in the supplemental material. The model-based 95% CI and parametric bootstrap 95% CI are introduced in Materials and Methods. The rate of stress development, λ, can also be understood as the rate at which the “unstress” state is lost, and the half-life (hours) of the unstressed state can be calculated simply by ln(2)/λ and is shown in parentheses following the estimates of λ.