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. 2017 Nov 22;37(47):11323–11334. doi: 10.1523/JNEUROSCI.2113-17.2017

Figure 8.

Figure 8.

a, Diagram illustrating (1) innervation of cornea by small-diameter axon nociceptors, (2) the lanceolate endings and large-diameter axons of mechanoreceptors surrounding hair cells, and (3) the stretch-sensitive large-diameter axons of proprioceptors that form a muscle spindle as the axon spirals around intrafusal muscle fibers. b, Corneas from 6-week-old control Sptan1f/f and αII spectrin-deficient Avil-cre; Sptan1f/f mice immunostained against Tuj1. Scale bar, 1 mm. c, The percentage of cornea that is Tuj1+. N = 4 corneas. Data are mean ± SEM. d, Hair follicles in skin from 2-month-old control Avil-cre; Sptan1f/+;ChR2-EYFP and sensory neuron-specific αII spectrin-deficient Avil-cre; Sptan1f/f;ChR2-EYFP mice were immunostained against GFP. Scale bar, 20 μm. e, The percentage of hair follicles with intact lanceolate endings in Avil-cre; Sptan1f/+;ChR2-EYFP (f/+) and Avil-cre; Sptan1f/f;ChR2-EYFP (f/f) mice. N = 3, 2-month-old animals. Data are mean ± SEM. Intact: ***p = 1.83392E-05, t(4) = 23.85; disrupted: ***p = 1.94369E-05, t(4) = 23.50. f, Gastrocnemius muscles from 2-month-old control Avil-cre; Sptan1f/+;ChR2-EYFP and sensory neuron-specific αII spectrin-deficient Avil-cre; Sptan1f/f;ChR2-EYFP mice were immunostained using antibodies against GFP and S46. Muscle spindles coil around S46-labeled intrafusal muscle fibers. Scale bar, 50 μm. g, The percentage of intact and disrupted muscle spindles in Avil-cre; Sptan1f/+;ChR2-EYFP (f/+) and Avil-cre; Sptan1f/f;ChR2-EYFP (f/f) mice. N = 3, 2-month-old animals. Intact: ***p = 5.2687E-05, t(4) = 18.28; disrupted: ***p = 0.0007, t(4) = 9.353; none: p = 0.0544, t(4) = 2.678.