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. 2017 Oct 23;89(22):12342–12350. doi: 10.1021/acs.analchem.7b04293

Table 1. Syntax of the Motif Language.

rule examples comments
standard glycan terms are accepted Galb1–4(Fuca1–3)GlcNAcb indicates the Lewis X motif
  Fuca1–3(Galb1–4)GlcNAcb indicates same motif; either branch may be enclosed
  standard abbreviations for monosaccharides
  “a” and “b” for α and β, linkages separated by dash or comma
  case insensitive
angled brackets specify details about the linkage or monosaccharide Neu5AcA2-<3or6>GalB1–4<3f6f>GlcNAcB1–2<4f;6f>ManA indicates Neu5Ac either 3′- or 6′-linked to an unbranched LacNAc
  <T>Gal indicates terminal galactose
  the “f” modifier specifies a location as unoccupied (free)
  an unspecified location indicates occupied or unoccupied
  the OR operator can be used to designate multiple options of linkage or monosaccharide
  the XOR operator can be used to indicate multiple options of linkage, where all other options of linkage are unoccupied (free)
  commas or semicolons can be included for clarity so that <3f6f>, <3f,6f>, and <3f;6f> are equivalent
  the “T” modifier is shorthand for all nonreducing locations being unoccupied (<2f3f4f6f> for hexose and <8f9f> for neuraminic acids)
special monosaccharide terms are available Any??-<3OR6>Galb1–4<3f;6f>GlcNAcb1–2<4f;6f>Mana indicates a single monosaccharide extension linked either 3′ or 6′ to an unbranched LacNAc
  Any??-<3OR6>Galb1–4<3f;6f>GlcNAcb1-?Mana indicates the same but with any linkage to mannose:
Fuca1-<2or3>Any?1-?Skip??-3(Fuca1-<2or3>Any?1-?Skip??-6)Galb indicates a fucose anywhere on both a 3′ and a 6′ extension of Gal
  the “Any” wildcard is for unspecified monosaccharides
  the “?” wildcard is for unspecified linkages
  the “Skip” modifier indicates a gap of any kind
  the “Skip” modifier encompasses lengths of zero, or no gap
double-angled brackets link complete terms with logical operators ≪ <2f;3f;4f;6f>Glca ≫ OR ≪ Mana ≫ indicates either terminal α-glucose or α-mannose
  ≪ Fuca ≫ 2 indicates two or more fucose
≪ Fuca1-<2or3or4>Any ≫ AND NOT ≪ Fuca ≫ 2 AND ≪ Siaa ≫ indicates a single fucose with at least one sialic acid
≪Fuca≫ AND NOT ≪Fuca≫ 2 AND NOT k OR ≪ Fuca1–2Galb1–3GalNac ≫ ≫ indicates a single fucose and excluding two other motifs
≪Motif1≫ AND NOT ≪Motif1≫ 2 AND NOT ≪Motif 2≫ uses Motif IDs as shorthand
  embedded double-angled brackets are supported
  the AND, OR, and NOT operators are supported