Figure 1. Technical Approach for Isolating and Characterizing Neutralizing Human Monoclonal Antibodies.

(A) Dielectrophoresis: physical apposition of myeloma cells (large) with human B cells (small) using delivery of an alternating electrical current causes “pearl-chaining” distribution. The process is based on an electrical principle, termed dielectrophoresis, in which force is exerted on a particle when it is subjected to a non-uniform electric field.

(B) Workflow for generation of human mAbs by generating clonal hybridoma cells from primary human B cells.

(C) Immunostaining of large plaque (top) or small plaque (bottom) variants of RSV is used to quantitate residual live virus precisely in plaque reduction neutralization tests.