Table 1.

Primer sequences and amplification programs.

Gene	Primer sequence	Conditions	Product size (bp)
IL-6	5′-ATGAACTCCTTCTCCACAAGCGC-3′ 5′-GAAGAGCCCTCAGGCTGGACTG-3′	5″at 95°C, 13″ at 56°C, and 25″at 72°C for 40 cycles	628
IL-8	5′-ATGACTTCCAAGCTGGCCGTG-3′ 5′-TGAATTCTCAGCCCTCTTCAAAAACTTCTC-3′	5″at 94°C, 6″ at 55°C, and 12″at 72°C for 40 cycles	297
IL-1β	5′-GCATCCAGCTACGAATCTCC-3′ 5′-CCACATTCAGCACAGGACTC-3′	5″at 95°C, 14″ at 58°C, and 28″at 72°C for 40 cycles	708
TGF-β	5′-CCGACTACTACGCCAAGGAGGTCAC-3′ 5′-AGGCCGGTTCATGCCATGAATGGTG-3′	5″at 94°C, 9″ at 60°C, and 18″at 72°C for 40 cycles	439
IL-1α	5′-CATGTCAAATTTCACTGCTTCATCC-3′ 5′-GTCTCTGAATCAGAAATCCTTCTATC-3′	5″at 95°C, 8″at 55°C, and 17″at 72°C for 45 cycles	421
TNF-α	5′-CAGAGGGAAGAGTTCCCCAG-3′ 5′-CCTTGGTCTGGTAGGAGACG-3′	5″at 95°C, 6″ at 57°C, and 13″at 72°C for 40 cycles	324