Fig. 5.

Evaluation of EGFR-SPIO nanoparticle targeting efficiency in GBM cell lines. a The targeting efficiency of EGFR-SPIO nanoparticles was quantified using flow cytometry. Resuspended U-87MG, U-251, and DBTRG-05MG GBM cells were subjected to flow cytometry analysis with EGFR-SPIO nanoparticles or the anti-EGFR antibody and goat anti-human AF-488 antibody. b Evaluate targeting specificity of EGFR-SPIO nanoparticles by IF staining. U-87MG and U-251 cells were fixed and incubated with empty media (negative control, NC), lipid SPIO nanoparticles, EGFR-SPIO nanoparticles, or the cetuximab for positive control (EGFR PC). After PBS wash, the specimens were incubated with goat anti-human AF-488 antibody to detect the presence of cetuximab. Green, cetuximab; blue, DAPI. Scale bar, 20 µm