Fig. 6.

Evaluation of the presence of SPIO nanoparticles in GBM cell lines. a Prussian blue staining for SPIO nanoparticles in GBM cell lines. U-87MG and U-251 cells were incubated with control media, media containing unconjugated lipid SPIO nanoparticles or EGFR-SPIO nanoparticles (100 ng/ml) for 24 h before Prussian blue and nuclear fast red staining. Scale bar, 20 µm. b Lipid SPIO or EGFR-SPIO nanoparticle sorption was detected in U-87MG or U-251 MG cells through TEM. The indicated GBM cells were exposed to EGFR-SPIO or unconjugated lipid SPIO nanoparticle at 0.1 mg/ml for 2 or 24 h before subjecting them to TEM. 20,000× magnification; scale bar, 200 nm; arrowhead, SPIO cluster; red dashed-line box, area with 50,000× magnification; scale bar, 20 nm