Fig. 5.

The CHOP production with the optimum recombinant E. coli. a The additive effect of repressing putA and overexpressing putP on CHOP production. Results are expressed as mean standard error of the mean (n = 3). Significance levels of Students t test: *P < 0.01, **P < 0.001; b the CHOP production and l-proline consumption by resting cells of E. coli BL21/pET28a-SmP4H-putP+anti-putA; c The synthesis of CHOP by the resting cells of BL21/pET28a-SmP4H-putP+anti-putA under the optimal reaction condition with a fed batch strategy