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. 2017 Nov 22;17:208. doi: 10.1186/s12886-017-0606-0

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Morphological changes induced by cyclic mechanical stretch. a-f Phalloidin staining of RPE cells at different time points; g The spreading cell ratio at different time points (red fluorescence: phalloidin staining; and blue fluorescence: DAPI nuclear staining). The morphological change of RPE cells was dependent on the duration of the stretch. At 14 h of cyclic stretch, the spreading cell ratio started to decrease sharply (p < 0.01). At 6 h after withdrawal of the stretch (R6h), the spreading cell ratio was significantly higher than at 14 h and 20 h (p < 0.01)