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. 2017 Nov 22;17:208. doi: 10.1186/s12886-017-0606-0

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Western blotting analysis of cytoskeleton regulatory protein expression. a Coloured bands of integrin signalling pathway-related proteins. b-d Expression levels of integrin, phosphorylated FAK397 (pFAK397), and phosphorylated Rac1/cdc42 (pRac/cdc42). The integrin expression (b) remained almost unchanged during the first 14 h of stretch, but started to decrease significantly at 20 h (p < 0.01). The expression of both pFAK397 (c) and pRac/cdc42 (d) was significantly up-regulated in the first 14 h of stretch (p < 0.05), but began to decrease at 20 h (p < 0.01)