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. 2017 Nov 22;17:218. doi: 10.1186/s12866-017-1129-9

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Persister cells from biofilms treated with combinations of antibiotics. Strains USA300 LAC, msaABCR deletion mutant, and the complementation were allowed to form biofilm on intravenous catheters for 24 h and then treated with combined antibiotics. a. daptomycin/rifampicin (80× MIC), b. vancomycin/rifampicin (40× MIC), c. linezolid/rifampicin (320× MIC), d. daptomycin/gentamicin (40× MIC), e. vancomycin/gentamicin (20× MIC), f. linezolid/gentamicin (20× MIC). These results represent the means of three independent experiments performed in triplicate. Error bars represent SE. Student’s t-test and one-way ANOVA (OriginPro) followed by Tukey’s test was used to compare the results of the wild-type to mutants at respective designated time points (* p-value <0.05, ** p-value <0.005)