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. 2017 Nov 22;17:218. doi: 10.1186/s12866-017-1129-9

Fig. 9.

Fig. 9

Comparative gene ontology analysis of msaABCR transcriptomics by for RNA-sequencing using Illumina- HiSeq 2000. The data was aligned by using Tophat-2.0.8b [50, 51]. Cufflinks-2.1.1 [52, 53] was used to measure the transcript level. Cuffdiff was used to determine expressed transcripts and measure the differential expression of genes in the msaABCR deletion mutant compared to wild type USA300 LAC. All the genes that are differentially expressed greater than 3-fold were considered significant and were analyzed by web-based GO analysis tool (Comparative GO) [54]