Table 4.
Comparison of the rate of persister cell formation between the msaABCR deletion mutant and wildtype USA300 LAC
| Fold change in persister cells formation (mutant vs. wildtype) | |||
|---|---|---|---|
| Antibiotics | Exponential Growth phase | Stationary Growth phase | Biofilm |
| DAP | UD** | - 2.15 | - 747.11** |
| VAN | - 23.82** | - 1.40 | - 2.8X104** |
| RIF | - 54.16** | - 1.66 | - 19.56 |
| LIN | - 1.18 | - 44.67** | - 14.24** |
| GEN | - 12.50** | UD** | - 2.33 |
| DAP + RIF | - 25.03** | - 23.02** | UD** |
| VAN + RIF | - 96.12** | - 4.04 | - 32.35** |
| LIN + RIF | 1.47 | - 3.35 | - 4.32 |
| DAP + GEN | - 55.30* | - 53.88** | - 0.73 |
| VAN + GEN | - 13.23** | - 0.88 | - 406.66** |
| LIN + GEN | + 14.43** | - 9.43** | - 37.50** |
DAP: daptomycin, VAN: vancomycin, RIF-rifampicin, LIN: linezolid, GEN: gentamicin, UD: undetectable persister cells in the msaABCR deletion mutant
Statistically significant (* p-value <0.05, ** p-value <0.005): The difference in CFUs observed in the mutant cells Vs wild type cells was analyzed by students T-test to measure the statistical significance. All the CFUs experiments that involved the biofilm cells were analyzed by one-way ANOVA followed by post-hoc Tukey’s HSD test to measure the statistical significance