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. Author manuscript; available in PMC: 2017 Nov 23.
Published in final edited form as: Cancer Res. 2016 Dec 6;77(4):982–995. doi: 10.1158/0008-5472.CAN-16-1589

Figure 1.

Figure 1

RB loss promotes cancer metastasis. A, RT-PCR analysis of RB, GAPDH mRNA, RB, and lamin B immunoblot in shCon and shRB LNCaP and PC3 cells. B, Confocal microscopic images of F-actin, pRb, and DAPI immunofluorescence in shCon and shRB LNCaP and PC3 cells. C, Confocal microscopic images of E-cadherin in shCon and shRB PC3 and LNCaP cells (left) and confocal microscopic images of vimentin in shCon and shRB PC3 cells (right). D, Graphic representation of quantitative migration kinetics with noncoated Boyden chamber and cell invasion kinetics with Matrigel-coated Boyden chamber in RB-proficient and -deficient PC3, PC3-ML, and LNCaP cells. E, Graphic representation of tumor luminescence from shCon and shRB PC3-ML tumor metastases in male SCIDs, with representative images (left and right). F, hematoxylin and eosin (H&E) and pRb staining of RB-proficient and -deficient PC3-ML tumor metastases at ×200 and ×400 (right). Each group contained a minimum of 6 animals. Each data point is a mean ± SD from three or more independent experiments. **, P < 0.05 was considered as statistically significant. Scale bar, 50 μm.