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. Author manuscript; available in PMC: 2017 Nov 23.
Published in final edited form as: Cancer Res. 2016 Dec 6;77(4):982–995. doi: 10.1158/0008-5472.CAN-16-1589

Figure 3.

Figure 3

RB/E2F complex transcriptionally regulates RHAMM. A, Schematic illustration shows the location of three putative RB/E2F–binding sites on the RHAMM promoter. B, Docking model shows the RB/E2F binding region on the RHAMM promoter. C, Anti-RB (top) and anti-ACH4 (bottom) chromatin immunoprecipitation (ChIP) analysis at the RB/E2F–binding site II on the RHAMM promoter. shCon and shRB PC3 cells were treated with either DMSO or PD 0332991. D, Transcription analysis of RHAMM promoter via luciferase assay in shCon and shRB PC3 cells in response to PD 0332991 or DMSO. E, RB-specific transcription activation of RHAMM promoter and known RB target promoter MCM7 in RB-deficient Saos2 cells in response to ectopic expression of RB-cDNA or vector control. Each data point is a mean ± SD from three or more independent experiments. **, P < 0.05 was considered as statistically significant.