Figure 6. Localization of FITC-labeled peptides from various GPCRs used in binding studies and their relation to previously identified CTS motifs.

The schematic drawing of the analyzed GPCRs shows the structural elements: NT: N-terminal extracellular domain, TM: transmembrane helices, third intracellular loop and C-terminal cytosolic region). Peptides (for their full sequences see Table 1) were designed based on the results of the peptide microarray (Figure 4 and Figure 4—figure supplement 1), and were tested for binding to the core BBSome. Black blocks indicate the approximate position of the peptides relative to the primary sequence, with the corresponding names to the right. The sequences of polybasic regions in Smoothened and of known CTS motifs within the third loop and the C-terminal domain of each GPCR are highlighted in color.