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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Brain Res Bull. 2017 Sep 15;135:47–52. doi: 10.1016/j.brainresbull.2017.09.007

Figure 2. Black Gold II staining of myelin and gene expression levels of CNP and MBP.

Figure 2

Representative photomicrographs of coronal brain sections at the level of the fimbria demonstrate progressive demyelination of the corpus callosum after 6 weeks of cuprizone (CPZ) exposure. Black-Gold II staining of control (A), CPZ (B), CPZ + Baicalein (C). Myelin densities in the corpus callosum (D) were compared with controls and expressed as a percentage of the control value using the ImageJ analysis program. Data are mean ± SEM, n = 7. Scale bar for = 500 μm. Gene expression of CNP (E) and MBP (F) relative to PGK1, as determined by real-time PCR in the corpus callosum after 6 weeks of cuprizone exposure. Data are mean ± SEM, n = 5 (***p < 0.001, **p < 0.01 vs. controls; ##p < 0.01, #p < 0.05 vs. CPZ).