Figure 1.
Component of the thrombin generation assay. The generation of thrombin during is measured through the fluorometric reaction. The parameters are calculated as follows: (1) lag time (LT) – the time interval from the beginning of the reaction to the detection of thrombin by the fluorometric assay; (2) estimated peak thrombin concentration (PTC) – the highest concentration of thrombin generated in a single measurement; (3) time-to-peak thrombin concentration (TPTC) – the interval from the beginning of the reaction and the generation of the highest thrombin concentration at a specific measurement; (4) velocity index (VI) – the rate of thrombin generation during the reaction; and (5) endogenous thrombin potential (ETP) – the calculation of how much thrombin has been generated during the time of the reaction (60 min). The figure was reproduced from www.Diapharma.com with permission.