Fig. 6.

R-Ras promotes formation of lumenized functional blood vessels via Akt. a In vivo transduction and treatment schedule. One day after lentivirus injection into GC muscles, R-Ras KO mice were treated with or without Akt inhibitor MK2206 by gavage every 2 days. GC muscle were analyzed at day 14. b Immunofluorescence of GC muscle cross-sections to examine α-tubulin acetylation in the endothelium of intramuscular vessels. *Blood cells in the lumen. c CD31 and PODXL immunofluorescence of the GC muscles that received lentivirus injection for EC-specific R-Ras38V D64A or R-Ras38V expression and subsequently treated with Akt inhibitor MK2206. *p < 0.01, n.s., not significant. d Analysis of vessel perfusion in whole-mounted GC muscle fascicles. Yellow color indicates lectin perfused vessels. **p < 0.02. Scale bars, 10 µm (b), 25 µm (c), 50 µm (d)