Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Expression levels of eight hFGF21 fusion proteins in E. coli BL21(DE3). Expression of the fusion proteins was induced by the addition of 0.5 mM IPTG at 37°C (A) or 18°C (B). The arrows indicate migration of hFGF21 fused with each tag in a polyacrylamide gel. M, molecular weight markers; C, total cell protein before IPTG induction as a negative control; I, whole cells after IPTG induction; P, the pellet fraction after cell sonication; S, the supernatant after cell sonication.
