Figure 4.

Effect of the down regulation of CK2 on IR-induced apoptosis. (A) A549 or H460 cells were pretreated with DMSO or 25 μM Quinalizarin for 24 h, then exposed to 0 or 4 Gy X-ray radiation. After 24 h, the cells were collected, resuspended with binding buffer and then stained with Annexin-V-PE and 7-AAD. Finally, apoptosis was measured by flow cytometry. The apoptosis rates were calculated. (**p < 0.01, ***p < 0.001) The mean ± S.D. was calculated for three independent experiments. (B) Cells were pretreated with DMSO or 25 μM Quinalizarin for 24 h, then exposed to 0 or 4 Gy X-ray radiation. After 24 h, the cells were harvested and total proteins were extracted. The protein level of PARP and P53 was detected by Western blot. (C) Cells were transfected with negative control siRNA or si-CK2α, after 48 h cells were exposed to 0 or 4 Gy X-ray radiation. 24 h later, the cells were collected and apoptosis was measured by flow cytometry. The apoptosis rates were calculated. (**p < 0.01, ***p < 0.001) The mean ± S.D. was calculated for three independent experiments.