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. 2017 Nov 23;7:16133. doi: 10.1038/s41598-017-16325-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of LipA from Xanthomonas axonopodis pv. citri strain 306 pathotype A. Representative results of (a) western-blot of the protein encoded by XAC_RS02605 expressed in E. coli DH5α. (b) Lipase activity around a colony of the transformed E. coli on solid LB medium containing tributyrin, whereas transformant with an empty vector (EV) did not show any activity (n = 3). (c) Esterase activity of secreted extracts measured by fluorescence emitted by degradation of 4-methylumbelliferone (MUB) (n = 4). (d) Hypersensitive response on tobacco leaf infiltrated with cell extracts of E. coli transformed with an empty vector (EV) or containing the XAC_RS02605 cassette (n = 3). (e) Confirmation of disruption of lipA in Xac306 by PCR amplification. (f) Lipase activity around Xac306 colonies (n = 3). (g) Esterase activity of secreted Xac306 extracts (n = 4). (h) Virulence assay on citrus leaf by infiltration of wild type Xac306 and mutant with inactivated lipA. See Methods for more details on assays.