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. 2017 Nov 23;8:1733. doi: 10.1038/s41467-017-01705-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Functional operation of microfluidic CTC isolation requires stabilized whole blood. a Representative images of the micropost array that performs size-based sorting (debulking) in the CTC-iChip. Blood storage in room temperature, even if treated with tiro-EDTA, results in aggregates that contain sheared DNA consistent with cell death and extracellular trap formation. Cold storage without tiro-EDTA leads to clots that contain densely packed platelets (CD61 staining) and intact cells. In both cases, rare cells are trapped within the aggregates. Cold storage with tiro-EDTA consistently permits clean processing (inset). b CTC isolation performance of the CTC-iChip in different storage conditions. All scale bars represent 50 μm. Box-and-whiskers plots show median, interquartile range, maxima, and minima. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 (one-way ANOVA followed by Tukey’s post test)