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. 2017 Nov 1;95(6):579–591. doi: 10.1007/s11103-017-0672-y

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — Accumulation of HSP22E/F during heat stress. a Immunoblot analysis of HSP22E/F accumulation during heat stress. cw15-302 cells grown at 25 °C were exposed to 39 °C for 120 min. Total proteins corresponding to 0.25 µg chlorophyll from cells harvested at the time points given were separated by SDS-PAGE and analyzed by immunoblotting using antisera against HSP22F and against CF1β as loading control. b Quantification of HSP22E/F signal intensities from (a). Signals were normalized to the maximal HSP22E/F levels reached during the time course. Error bars represent SD, n = 2