Fig. 6.

Verification of proteins co-precipitating with HSP22E/F by immunoblot analysis. Total soluble proteins were extracted from cells grown at 25 °C and shifted to 39 °C for 0 or 60 min. Extracts were supplemented with or without the homobifunctional crosslinker DSP prior to the immunoprecipitation of HSP22E/F. 0.3% of the input for immunoprecipitation and 5% of the immunoprecipitates were separated on a 12% SDS–polyacrylamide gel and analyzed by immunoblotting. The asterisk indicates a protein crossreacting with anti-RbcL antibodies