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. 2017 Nov 23;7:16153. doi: 10.1038/s41598-017-16543-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phenotype of inguinal subcutaneous white adipose tissue in 4-month-old Egr1 ^−/− mice. (A) Pictures of fat pads (SC-WAT) from 4-month-old Egr1 ^+/+ and Egr1 ^−/− mice. Scale bars: 5 mm. (B) Weight in grams of SC-WAT of 4-month-old Egr1 ^+/+ and Egr1 ^−/− mice. The graph shows mean ± standard deviations of 6 Egr1 ^+/+ fat pads and 8 Egr1 ^−/− fat pads. (C) Weight in grams of 4-month-old wild-type and mutant mice. The graph shows means ± standard deviations of 4 Egr1 ^+/+ and 4 Egr1 ^−/− mice. The p-value was obtained using the Mann-Whitney test. Asterisk indicates the p-value *P < 0.05. (D) SC-WAT of 1-month-old mice was longitudinally sectioned. 6 µm sections were hybridized with the DIG-labeled antisense probe for Egr1 (blue). Arrow a points Egr1 expression in blood vessels. Arrows b and c indicate Egr1 expression in white adipocytes. Scale bars: 50 µm.