Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 23;8:1749. doi: 10.1038/s41467-017-01981-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commonslicense, unless indicated otherwise in a credit line to the material. If material is not included in the article’sCreative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — Experimental validations of the computationally predicted smORFs. a rRibo-seq and LTM-based TI-seq RPF count profiles in HEK293 cell line for a predicted uORF in 5′UTR of EIF5. b FLAG-tagged uORF within the context of the host mRNA was ectopically expressed and translation of the predicted polypeptide was detected by western blot with an anti-FLAG antibody. β-actin protein was used as internal control for western blot analysis. c rRibo-seq and LTM-based TI-seq RPF count profiles for a predicted ORF encoded by lncRNA DANCR. d FLAG-tagged smORF within the context of host lncRNA was ectopically expressed and translation of the predicted polypeptide was detected by western blot with an anti-FLAG antibody. e rRibo-seq and LTM-based TI-seq RPF count profiles for a predicted ORF encoded by an intron of BLOC1S3. f FLAG-tagged intronic smORF within the context of the host mRNA was ectopically expressed and translation of predicted polypeptide was detected by western blot with an anti-FLAG antibody