Table 1.
Figures of merit for determination of multiple substances in blood spots by SPME-CAN methodology via CBS-MS/MS.
| Compound | Log Pa | Protein binding (%)b | LOQ, | LOQ, | Accuracy (n = 4), % | Precision (n = 4), % | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Spot-alone | SPME-CAN | 3 ng/mL | 40 ng/mL | 80 ng/mL | 3 ng/mL | 40 ng/mL | 80 ng/mL | |||
| ng/mL | ng/mL | |||||||||
| Salbutamol | 0.44 | — | 10 | 2.5 | 113.6 | 81.7 | 86.3 | 7.1 | 7.5 | 12.7 |
| Morphine | 0.99 | 30–40 | 10 | 2.5 | 118.6 | 86.9 | 87.2 | 6.5 | 6.3 | 3.6 |
| Oxycodone | 1.04 | 45 | 10 | 2.5 | 110.7 | 81.9 | 93.6 | 6.9 | 11.4 | 5.6 |
| Codeine | 1.20 | 7–25 | 2.5 | 0.5 | 94.2 | 80.8 | 93.7 | 6.5 | 4 | 3.2 |
| Cocaine | 1.97 | — | 2.5 | 0.25 | 92.9 | 86.4 | 96.1 | 3.5 | 2.4 | 1.2 |
| Methamphetamine | 2.23 | — | 10 | 0.5 | 90.1 | 84.3 | 95.7 | 2.9 | 2.1 | 1.6 |
| Bisoprolol | 2.30 | 30 | 1 | 0.5 | 95.8 | 87.7 | 95.6 | 4.2 | 3.1 | 7.7 |
| Diazepam | 2.63 | 98.5 | 2.5 | 0.5 | 95.5 | 80.5 | 93.4 | 3.7 | 4.5 | 2.3 |
| Carbamazepine | 2.77 | 76 | 1 | 0.25 | 113.2 | 90.7 | 95.3 | 1.7 | 1.8 | 2.9 |
| Clenbuterol | 2.94 | — | 1 | 0.5 | 95.9 | 83.3 | 96.4 | 2.8 | 1.7 | 0.9 |
| Lorazepam | 2.98 | 89–93 | 10 | 2.5 | 128.4 | 91.5 | 103.6 | 8.5 | 8.7 | 16.4 |
| Propranolol | 3.03 | >90 | 5 | 0.5 | 92.0 | 83.2 | 92.6 | 1.8 | 1.9 | 1.9 |
| Citalopram | 3.58 | 80 | 2.5 | 0.5 | 95.7 | 85.3 | 94.6 | 4.5 | 2.5 | 2.4 |
| Fentanyl | 4.12 | 80–85 | 2.5 | 0.25 | 93.2 | 85.7 | 95.2 | 2.3 | 1.7 | 0.8 |
| Methadone | 4.14 | 85–90 | 1 | 0.25 | 96.2 | 92.8 | 93.5 | 2.6 | 1.5 | 0.9 |
| Buprenorphine | 4.53 | 96 | 1 | 0.5 | 98.4 | 85.8 | 97.8 | 7.2 | 8.3 | 4.1 |
| Sertraline | 5.06 | 98 | 5 | 0.25 | 96.5 | 87.1 | 95.1 | 2.4 | 8.5 | 3.3 |
a,bLog P and protein binding values were obtained from www.drugbank.ca.