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. 2017 Nov 23;7:16095. doi: 10.1038/s41598-017-16453-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Visualization and analysis of MEPs with a custom-made MATLAB® algorithm. (A) Both ipsi- and contralateral MEPs were recorded for left and right motor cortex stimulation. Per limb, 4 stimuli were given and consequently 4 MEPs were recorded. Stimulation intensities ranged between 0–60 V for the forelimbs and 0–100 V for the hindlimbs. Based on the guidelines, the motor threshold was defined as the lowest stimulus intensity of motor cortex stimulation required to elicit at least 3 MEPs of similar shape⁵. (B) At motor threshold, response onset (point 1), maximal and minimal peaks (point 2 and 3) and the end of the response (point 4) were defined by 3 independent and blinded observers. These measurements were then used to calculate latency, area-under-the-curve, peak-to-peak and duration of each MEP at the motor threshold.